MersV1, Main, Exploration, bibRecord, 003080

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence.

Identifieur interne : 003080 ( Main/Exploration ); précédent : 003079; suivant : 003081

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence.

Auteurs : T J V. Prazeres [Portugal] ; A M Santos ; J M G. Martinho ; A. Elaïssari ; C. Pichot

Source :

Langmuir : the ACS journal of surfaces and colloids [ 0743-7463 ] ; 2004.

RBID : pubmed:15274592

Descripteurs français

English descriptors

KwdEn :
- Acrylic Resins (chemistry), Adsorption, Light, Molecular Structure, Oligonucleotides (chemistry), Polymethyl Methacrylate (chemistry), Scattering, Radiation, Sensitivity and Specificity, Spectrometry, Fluorescence (methods), Surface Properties, Temperature.
MESH :
- chemical , chemistry : Acrylic Resins, Oligonucleotides, Polymethyl Methacrylate.
- methods : Spectrometry, Fluorescence.
- Adsorption, Light, Molecular Structure, Scattering, Radiation, Sensitivity and Specificity, Surface Properties, Temperature.

Abstract

The adsorption of a rhodamine X labeled oligonucleotide composed of 25-mers of thymine (dT(25)-ROX) onto the thermosensitive shell of PMMA/PNIPAM core-shell latex particles was studied at 22 and 40 degrees C, below and above the T(VPT) (volume phase transition temperature) of the PNIPAM shell, respectively. The experimental binding isotherms were well fitted with the cooperative Hill model. The Hill coefficient is lower than 1 at both temperatures showing that the adsorption is anticooperative. The polarity of the shell was probed by both the lifetimes and solvatochromic shifts of the zwitterionic form of rhodamine X. For temperatures below the shell T(VPT) has a polarity similar to that of water, while for temperatures above the transition the polarity is equivalent to that of a water/dioxane mixture with 30% (v/v) water.

DOI: 10.1021/la049609u
PubMed: 15274592

Affiliations:

Portugal

Le document en format XML

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<front><div type="abstract" xml:lang="en">The adsorption of a rhodamine X labeled oligonucleotide composed of 25-mers of thymine (dT(25)-ROX) onto the thermosensitive shell of PMMA/PNIPAM core-shell latex particles was studied at 22 and 40 degrees C, below and above the T(VPT) (volume phase transition temperature) of the PNIPAM shell, respectively. The experimental binding isotherms were well fitted with the cooperative Hill model. The Hill coefficient is lower than 1 at both temperatures showing that the adsorption is anticooperative. The polarity of the shell was probed by both the lifetimes and solvatochromic shifts of the zwitterionic form of rhodamine X. For temperatures below the shell T(VPT) has a polarity similar to that of water, while for temperatures above the transition the polarity is equivalent to that of a water/dioxane mixture with 30% (v/v) water.</div>
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Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence.

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki

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